Patient-specific and unique DMRs. (A) MeD-seq data was pooled per patient to call interpatient-specific and unique TSS and CpG island DMRs that show differential methylation consistent in all tested tissues. (B) Overview showing the number of patient-specific and patient-unique TSS and CpG island DMRs identified. TSS and CpG island DMRs with a read count of more or less than 200 and 1000, respectively, are shown separately. (C) MeD-seq tracks showing patient-specific differential methylation of the ADAM3A TSS and patient-unique methylation of two CpG islands. (D) Patient-specific TSS DMRs were determined for blood that showed consistent DMRs in other organs tested. (E) Percentage of blood-specific TSS DMRs also present in liver, ovary, and thyroid in patient comparisons. (F) Percentage of blood-specific CpG island DMRs also present in liver, ovary, and thyroid in patient comparisons. (G) MeD-seq tracks showing blood-specific DMRs present in all organs (left), no other tested organ (ZNF529, middle), or some organs (DUSP22, right). (H) Percentage of CpG island DMRs present both in blood and buccal and also present in liver, ovary, and thyroid. (I) Correlation plots showing blood TSS DMRs also present in buccal (top) and blood CpG island DMRs also present in buccal (bottom) for patients 3 and 4.
