Recovery rates of DNA (A) and RNA (B,C) by the SIDR method. (A) The efficiency of DNA recovery by the SIDR method was estimated by real-time PCR targeting the LINE-1 locus. (B) The efficiency of cytoplasmic RNA recovery by the SIDR method was estimated by real-time PCR targeting GAPDH, CDKN1A, PSMC4, 18S rRNA, and 5S rRNA. (C) The additional three transcripts reported to be enriched in the nucleus were assessed by real-time PCR targeting GATA6, APBB2, and SVIL. Nucleic acids were extracted from 10 MCF7 cells. FD and FR refer to genomic DNA and total RNA, respectively, fractionated by the SIDR method. The amount of DNA in FR and of RNA in FD indicates the amount of residual contamination in the counterpart fractions due to incomplete separation. The amounts of nucleic acids in each fraction were normalized to those in the whole-cell lysates of 10 MCF7 cells. Error bars represent the SEM.
