Establishment of MMR-deficient A. thaliana mutation accumulation (MA) lines and overview of mutations identified. (A) Steps in the generation of MA lines: (1) preparation of Generation 0 (G0) Atmsh2-1 Ancestor; (2) determination of Atmsh2-1 Ancestor whole-genome sequence; (3) creation of independent MA lines by self-pollination of Atmsh2-1 Ancestor and subsequent single-seed descent; (4) recovery of 40 fifth generation (G5) MA line plants; and (5) determination of whole-genome sequence of eight G5 MA line plant samples. (B) Overview of total mutations accumulated in all eight G5 Atmsh2-1 MA line plant samples. Deletions are 1–5 bp in size; insertions are 1–3 bp in size; SNVs are single-nucleotide variants (single-nucleotide substitutions).
