Overexpression of H3S10A in ESCs promotes H3K9me2 accumulation in early-replicating regions. (A) ChIP enrichment of YFP (RPKM) around transcription start sites (TSS ±500 bp) in WT and S10A H3.3-YFP-expressing lines, clustered by genic expression quartiles. (B) Genome browser screen shot spanning Cpeb4 to Fbxw11 loci on Chromosome 11 (31,696,800–32,668,200) with YFP and H3K9me2 ChIP enrichment in H3.3-YFP WT and S10A mutant lines presented, as well as previously published RT (Yokochi et al. 2009) and H3.3-HA (Elsässer et al. 2015) tracks. (C) Histogram showing the global density distribution of H3K9me2 in H3.3-YFP WT and S10A lines. (D) 2D scatterplot comparing the gain or loss (z-score) of H3K9me2 in the S10A.2 mutant line with WT H3K9me2 enrichment levels (RPKM). (E) Comparison of YFP incorporation levels with the change in H3K9me2 enrichment in H3.3-YFP S10A.2 relative to WT cells (z-score). Heat map and data-point size represent the range of WT H3S10ph enrichment levels. Note that gain of H3K9me2 occurs predominantly within regions that are enriched for H3S10ph in WT ESCs.
