Identification, characterization, and regulation of miRNA editing sites. (A) The distributions of RNA editing levels for known and novel sites in different species. For this analysis, we used the representative editing level of each editing site, which is the maximum editing level across all samples we profiled. (B) Metagene profiles depicting the editing site distribution across pri-miRNAs. The structure predicted using dme-mir-2492 precursor and the flanking 150-nt sequence is used as the representative pri-miRNA secondary structure. Percentage of As which were edited at each position is indicated by color. (C) Box plot showing the number of editing sites per pre-miRNA in human, mouse, and D.mel. P-values were calculated using Wilcoxon test: (*) P < 0.05; (**) P < 0.01; (***) P < 0.001. (D) The proportion and mean representative editing level comparison between editing sites with different complementary nucleotides. Schematic diagram was used to explain how we categorized the editing sites. Edited As are highlighted in red. Others, editing sites that cannot be categorized into the previous 5 types. The mean editing levels for each type were shown as the line plot. The color codes for species are the same as in C.
