Statistically robust methylation calling for whole-transcriptome bisulfite sequencing reveals distinct methylation patterns for mouse RNAs

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Figure 4.
Figure 4.

LC-MS/MS analysis of RNA samples from mouse ES cells that were subjected to multistep mRNA enrichment. (A) Basic outline of the mRNA enrichment protocol. (B) Relative amounts of mRNA and rRNA as determined by RNA-seq. The proportion of tRNA reads was <1% for all samples. (C) Modification analysis of m5C, hm5C, m26A, m6A, and m1A content, relative to A content.

This Article

  1. Published in Advance July 6, 2017, doi: 10.1101/gr.210666.116 Genome Res. 27: 1589-1596

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