Statistically robust methylation calling for whole-transcriptome bisulfite sequencing reveals distinct methylation patterns for mouse RNAs

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Figure 2.
Figure 2.

WTBS of mouse ES cell mRNA. (A) Frequency of bisulfite nonconversion (resp. methylation) ratios in mRNA. (B) Estimated Poisson rate λp/coverage as a function of coverage, as well as corresponding boxplot. (C) Bisulfite nonconversion ratio (x-axis) and −log of methylation P-values (y-axis) for 53,510 cytosines passing the 0.2 ratio threshold. Color indicates density (count of points per symbol area). (D) Cytosine position and methylation tracks for a representative mRNA transcript (NM_007984) in three replicates.

This Article

  1. Published in Advance July 6, 2017, doi: 10.1101/gr.210666.116 Genome Res. 27: 1589-1596

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