Figure 3.
SL1 trans-spliced isoforms exhibit higher translational efficiencies than the non-trans-spliced isoforms of the same genes. (A) mRNAs with different numbers of ribosomes bound to them were fractionated by sucrose gradient centrifugation. (B) Isoform-specific qPCR primers were designed to quantify the concentrations of two isoforms. (C) The concentration ratio of two isoforms (SL1 trans-spliced/non-trans-spliced) was quantified in each fraction of fractions 6–14. The locally weighted scatterplot smoothed (LOWESS) lines are shown in blue. P-values were given by Spearman's rank correlation.
