Formation of ALS-associated protein aggregates is attenuated in specific yeast gene deletion strains. (A) Loss of specific yeast genes reversed the aggregation of the ALS-associated proteins. To observe protein localization, OPTN and ANG were cloned into pAG425GAL-ccdB-GFP and transformed into the deletion strains. Cells expressing OPTN-GFP or ANG-GFP were observed with fluorescence microscopy after 16 h of OPTN/ANG gene induction. (B) OPTN and ANG protein aggregation was cross-tested in representative yeast deletion strains of ANG and OPTN toxicity suppressors, respectively. (C) From the genetic interaction results of the cross-test, a network for the two ALS genes was constructed.
