Overexpression of selected OMIM genes induces toxicity and cytoplasmic aggregates in yeast. (A) In total, 1305 OMIM ORFs were cloned under the control of a galactose-inducible promoter in pAG425 vectors. pAG425Gal-OMIMs were individually transformed into yeast. Transformants were grown in SRaf-Leu medium for 16 h, spotted onto SD-Leu agar plates (OMIM expression “off”) or SGal-Leu agar plates (OMIM expression “on”), and incubated for 3 d. Shown are 10-fold serial dilutions starting with an equal number of cells expressing the 20 toxic OMIM genes. Nontoxic OMIM genes are not shown. (B) Yeast cells expressing the “C-terminal GFP-tagged OMIM” fusion proteins were visualized with fluorescence microscopy. GFP alone was distributed in the cytoplasm. Most of the toxic GFP-tagged OMIM proteins formed protein aggregates.
