A pre-MBT replication timing program anticipates initial zygotic transcription. (A) Replication timing values scaled to S-phase length (left) for pre-MBT (blue line) and ZTF cells (gray line) and scaled to normalized units (right) for pre-MBT biological replicates. (B) Pearson's correlation for genome-wide replication timing values from biological replicates of pre-MBT embryos. (C) Autocorrelation for biological replicates of pre-MBT replication timing data (blue lines) and randomly permutated data (black lines). (D) Replication timing of the genome overall (white), all genes (gray), the genes transcribed pre-MBT (blue), or the first genes transcribed after zygotic genome activation (first wave, red). ANOVA with Bonferroni post-hoc correction: (**) P < 0.0001; (*) P = 0.0067. Box plots show the median (line), 95% confidence interval (notch), 25th–75th percentile (box), and 10th–90th percentile (whiskers).
