DONSON expression in human and mouse brain development. (A) DONSON expression by in situ hybridization in a coronal section of a 9-wk-gestation human fetal brain. Expression is prominent in the neocortex subventricular zone, which contains progenitor cells, and in the cortical plate, where newly born neurons reside. Expression is also seen in the ganglionic eminences, which give rise to the basal ganglia and interneurons that migrate into the neocortex. Scale bar for main image (left) is 500 µm; scale bars for other images are 100 µm. Hybridization was performed with antisense probe 1 (see Supplemental Methods). (Ctx) Neocortex, (GE) ganglionic eminence, (v) ventricular zone, (sv) subventricular zone, (i) intermediate zone, (c) cortical plate, (m) marginal zone. (B) DONSON expression by in situ hybridization in a sagittal section of a 12-wk-gestation human fetal brain. Expression is prominent in the basal ganglia (BG) and the ventricular and subventricular zones and cortical plate of the neocortex (Ctx), mesencephalon (M, midbrain), and rhombencephalon (R, hindbrain). Scale bar for main image (left) is 1000 µm; scale bars for other images are 100 µm. Hybridization was performed with antisense probe 1. (C) DONSON expression by in situ hybridization in a coronal section of a hemisphere of a 20-wk-gestation human fetal brain. Expression is evident in the ventricular and subventricular zones, intermediate zone, and cortical plate of the neocortex (Ctx). Scale bar for main image (left) is 1000 µm; scale bar for other image is 100 µm. Hybridization was performed with antisense probe 2. (D) DONSON expression by in situ hybridization in a coronal section of an E15.5 mouse brain. Expression is evident in the ventricular (v) and subventricular zones (sv), intermediate zone (i), and cortical plate (c) of the neocortex. Scale bar for top and bottom images are 500 and 100 µm, respectively. Hybridization was performed with human antisense probe 3. For all above tissue sections, negligible signal was observed with sense sequence probes in adjacent sections (Supplemental Fig. 3D), confirming specificity of the antisense probe staining.
