Figure 2.
Concordance of mutations in bulk primary and metastatic tumors. (A,B) Scaled Venn diagrams reflect the total number of mutations (synonymous and nonsynonymous) identified by exome sequencing of the bulk flow-sorted tumor cells from the primary and metastatic tumors. (C,D) Dot plots showing the variant allele frequencies of the nonsynonymous mutations in the primary and metastatic tumors. (E) Targeted deep amplicon sequencing of the metastasis-specific mutations in the primary tumor and matched normal tissue. Significance of the mutations based on the variant read counts was determined using deepSNV and a Bayesian hypothesis test (Methods).
