Figure 5.
Sequence content of libraries obtained from extracts prepared with the four methods as inferred by qPCR and shotgun sequencing. (A) The sum of nucleotides in all library molecules relative to that of the best method. (B) The sum of ‘informative’ nucleotides in the libraries, i.e., the sum of nucleotides subsumed in library molecules ≥35 bp that produce sequence alignments, relative to that of the best method. Numbers for the libraries prepared from buffer exchange extracts were multiplied by 2.5 to compensate for the smaller volume of lysate used.
