Contacts with promoters and terminators of active genes best predict reporter expression. (A) Chromatin and conformational features are used individually to predict the expression level of the reporters. The feature with highest predictive power is the amount of contacts with terminators (i.e., the position of the 3′ end of each gene) of active genes, followed by contacts with promoters. Histone marks are indicated in orange, the mean expression of flanking genes in purple, and chromatin state models in pink. (B) Lasso multiple regression. By including more variables, the predictive power goes up to 0.33. Nineteen chromatin features are required to increase the predictive power from 0.25 to 0.29, and 89 are required to reach 0.32. This means that chromatin features are redundant and have multiple small effects.
