YY1-mediated developmentally regulated looping interactions form within a constitutive framework demarcated by CTCF. (A) Western blot analysis querying YY1 and GAPDH protein levels in NPCs exposed to nontargeting control and Yy1-targeting siRNA. (B) Gene-expression quantified by qPCR of the Yy1 gene in NPCs exposed to control and Yy1-targeting siRNA. (C) Zoom-in interaction score heatmaps of a loop between the Sox2 gene and an upstream enhancer (originally presented in Fig. 3K) in NPCs exposed to nontargeting control siRNA (left) and an siRNA targeting Yy1 (right). (D) Gene-expression quantified by qPCR of the Sox2 gene in NPCs exposed to control and Yy1-targeting siRNA. (E) Schematic depicting a CTCF-mediated constitutive interaction, present across all early stages of neural lineage commitment, and a YY1-mediated gene-enhancer interaction, present only in NPCs.
