Mechanisms of single-nick–induced PGE using ODN donors. (A) Repair of complementary strand nicks via SDSA. (B) Repair of nicks on the same strand via ssDI. Schematic elements are labeled as follows: (Top) genomic DNA, black; genomic lesions and DNA ends, hatched orange lines; ODNs, red; knock-in mutations, green; resection nucleases, yellow PAC-MAN; base-pairing, purple vertical lines; DNA synthesis, red dashed arrows; processing nucleases, yellow lightning bolts; (bottom) predicted SNP retention curves, red dashed line; predicted position of genomic lesions, orange vertical dashed line; position of the knock-in mutation selected for, green vertical solid line; regions with more than 50% coconversion frequency, black double-headed arrows; strandedness of DNA, S and AS; orientations of the DNA ends, 5′ and 3′.
