Nucleosomes are remodeled in a subset of genes during WT development. (A) Normalized read midpoint frequencies in growing-stage (black) and loose-mound–stage (red) WT cells for all 12,750 genes aligned to their ATG sites. (B) Normalized read midpoint frequencies in growing-stage (black) and loose-mound–stage (red) WT cells for all 12,750 genes aligned to the midpoint of the first defined nucleosome in growing WT cells. (Inset) Enlarged view of nucleosomes 4 and 5 to show the +3-bp NRL shift from growth to development. (C) Normalized read midpoint in growing-stage (black) and loose-mound–stage (red) WT cells for the 2856 developmentally remodeled genes aligned to the midpoint of the first defined nucleosome in growing WT cells. (D) Normalized read midpoint frequencies in growing-stage (black) and loose-mound–stage (red) WT cells for the 9894 non-remodeled genes aligned to the midpoint of the first defined nucleosome in growing WT cells. (Inset) Enlarged view of nucleosomes 4 and 5 to show the increased NRL from growth to development.
