Figure 3.
PRDM9-dependent H3K36me3 enrichment in class 1 sites is independent of double-strand break formation. Average and normalized read enrichment (RPKM) of H3K36me3 in B6 (blue), B6 Spo11KO (green), and RJ2 (red) mice. B6 and B6 Spo11KO mice have the same PRDM9 allele (Dom2). Read enrichments are centered on PRDM9 class 1, 2A, and 2B sites and normalized by subtracting the input read enrichment. Only peaks that overlapped with intergenic regions were considered for class 1 (826 and 3321 sites for B6 and RJ2, respectively) to avoid noise from the H3K36me3 signal specifically found at transcribed genes.
