Native and divergent CENH3 exhibit identical binding patterns. (A) Histogram depicting fold enrichment of CENH3 signal over negative control on A. thaliana Chromosome 5, with increasing magnification on the region with maximum signal. Colored bars parallel to the x-axis represent CEN180 repeats, colored by cluster. (B) Distribution of CENH3 ChIP signal across the six CEN180 clusters illustrated in shades of blue. Input in black is shown in comparison and represents abundance in the genome. (C) A. thaliana and Z. mays CENH3s form intermingled subdomains in interphase nuclei of wild-type A. thaliana expressing Z. mays CENH3. Analysis was performed by Structured Illumination Microscopy (SIM): (blue) counterstain with DAPI; (green) A. thaliana CENH3; (red) Z. mays CENH3. Area within the white square is enlarged. (D) Signal intensity of A. thaliana and Z. mays CENH3 measured in eight nuclei. Signal intensities of CENH3s are not significantly different at the 5% level by t-test.
