GR binds at corticosterone-increased binding sites and increases their accessibility. (A,B) Corticosterone-increased CREB1 binding sites show more GR binding than corticosterone-unaffected sites (measured by ChIP-seq tag density). (**) Statistical significance (P ≤ 0.0001). (C) The number of GR peaks in the vicinity of cort-unaffected or cort-increased CREB1 peaks was plotted as a function of distance from CREB1 peak center (plotted in 50-bp bins). (D,E) Corticosterone-increased CREB1 binding sites show more DNase I hypersensitivity than corticosterone-unaffected sites in livers from adrenalectomized mice treated with dexamethasone (dex, 1 mg/kg) for 1 h, DNase-seq data were generated and described in Grøntved et al. (2013) (measured by DNase-seq tag density). (**) Statistical significance (P ≤ 0.0001). (F,G) GR increases DNase I hypersensitivity at fasting-induced enhancers (measured by DNase-seq tag density). Adrenalectomized mice were treated with dexamethasone (dex, 1 mg/kg) for 1 h, DNase-seq data were generated and described in Grøntved et al. (2013). (**) Statistical significance (P ≤ 0.0001).
