Genome surgery using Cas9 ribonucleoproteins for the treatment of age-related macular degeneration

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Figure 4.
Figure 4.

Genome-wide target specificity of the Vegfa-specific Cas9 RNP revealed by Digenome-seq. (A) Genome-wide Circos plot (Krzywinski et al. 2009) showing in vitro cleavage sites. Human genomic DNA is shown in red, and RGEN-digested genomic DNA is shown in blue. (B) Sequence logo obtained using 42 Digenome-captured sites. (C) Off-target site validated in human ARPE-19 cells by targeted deep sequencing. The mismatched nucleotides are shown in red, and PAM sequences are shown in blue.

This Article

  1. Published in Advance February 16, 2017, doi: 10.1101/gr.219089.116 Genome Res. 27: 419-426

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