Relationship between Tel1- and Zip3-dependent control pathways. (A) Negative correlation between Spo11-oligo density and chromosome size requires Zip3 but not Tel1. zip3Δ data are from Thacker et al (2014). (B) Local domains of correlated behavior (as in Fig. 4D). Data for tel1Δ 4 h are reproduced from Figure 4D. See Supplemental Figure S4C for randomized controls. Data from a mutant lacking the transcription factor Bas1 serve as a negative control for this analysis, because this transcription factor affects DSB formation within a subset of individual hotspots rather than coordinately influencing local domains containing multiple hotspots (Zhu and Keeney 2015). (C–E) Spo11-oligo time courses as in Figure 1. Mean ± SD for four pairs of cultures is shown. Data from zip3Δ are reproduced from Thacker et al (2014) for comparison (see also Supplemental Fig. S4B).
