
Sizes of Spo11 oligos in Tel1 pathway mutants. (A) Radiolabeled, de-proteinized Spo11 oligos separated on a denaturing 15% polyacrylamide sequencing gel. Lanes are from a single gel with an intervening lane omitted. (M) 10-nt ladder. Note that oligos from lower-molecular-weight Spo11-oligo complexes are underrepresented because most of the shortest oligos (less than ∼12 nt) are lost upon ethanol precipitation. (B,C) Lane profiles from panel A.











