ORC-bound origins lose nucleosomes during the G2/M to G1 transition. (A) Average nucleosome occupancy (log2) within a 2-kb window of ACS for ORC-bound origins (left), ORC-unbound origins (center), and TTSs (right) in G1 (blue) and G2/M (red). The dotted line represents the midpoint of the ACS. (B) The difference in nucleosome occupancy (log2) between G2/M and G1 (G2/M-G1) for ORC-bound origins (left), ORC-unbound origins (center), and TTSs (right). (C) Heat maps of the changes in nucleosome occupancy from G2/M to G1 within a 2-kb window of the ACS for ORC-bound (left) and ORC-unbound (right) origins. Origins are ranked based on the degree of the changes in nucleosome occupancy. (D) The decrease in nucleosome occupancy between G2/M and G1 was correlated to origins classified by occupancy in G1. Low nucleosome occupancy class is shown in blue, mid nucleosome occupancy class is shown in red, high nucleosome occupancy class is shown in green, and ORC-unbound ACSs is shown in purple.
