The Med13l locus contains a required enhancer cluster and a dispensable super-enhancer. (A) Schematic representation of Med13l locus. Transcription factor-bound regions (red bars), MED1 and H3K27ac ChIP-seq and RNA-seq data obtained from the CODEX database are shown. Predicted enhancers (prEnh) and called super-enhancers (SEs) are shown in black. The deleted regions (ΔEC and ΔSE) are shown with a line that links the 5′ and 3′ gRNA targets. All data are displayed on the mm10 assembly of the UCSC Genome Browser. (B) Deletion of ΔEC but not ΔSE significantly affects the expression of the linked Med13l allele. Allele-specific primers detect 129 or Cast RNA in RT-qPCR from F1 ES, Δ129/+, and Δ+/Cast clones. Expression for each allele is shown relative to the total. Error bars represent SEM. Significant differences from the F1 ES values are indicated: (***) P < 0.001.
