Noncoding DHSs tested from ceh-13, icl-1, and pha-4 genes drive expression in transgenic C. elegans larvae and adults. Noncoding DHSs drive expression in diverse tissues of transgenic C. elegans. (A) ceh-13 first intron (A1) expresses in pharyngeal cells. (B) ceh-13 3′ distal (A2) expresses in uterus (likely uv2). (C) ceh-13 3′ more distal (A3) expresses in anterior body wall muscle. (D) icl-1 distal (B1) expresses in seam cells and hypodermis. (E) icl-1 intron (B3) expresses in intestine, body wall muscle, and hypodermis. (F) icl-1 promoter (B2) expresses in cells around the pharynx and intestine. (G) pha-4 upstream (C1) expresses in hypodermis. (H) pha-4 promoter (C2) expresses in uterus and vulva (likely vulC). In some animals, expression is observed in seam cells. (I) pha-4 promoter (C3) expresses in somatic gonad. (J) pha-4 downstream (C4) expresses in cells around the pharynx.
