Sex-biased miRNA expression in mouse, opossum, and chicken. Male-to-female (M:F) ratios were calculated for three male and three female replicates per tissue and the significance assessed with DESeq2 (Love et al. 2014), with Benjamini-Hochberg correction for multiple tests (Benjamini and Hochberg 1995) performed separately for somatic and gonadal samples. For display purposes, the log2-transformed M:F ratios were capped at −3 and 3, with more extreme ratios replaced by these values. Each data point corresponds to a multimap group (MMG) comprised of one or more mature miRNAs (see main text), and MMGs were considered sex-linked if they included at least one sex-linked member. Expression values correspond to the mean normalized counts provided by DESeq2. The most sex-biased miRNAs per tissue are listed in Table 1. The Z-linked and W-linked miRNA miR-122-5p is visible as the rightmost data point in the chicken liver panel. All data shown in this figure are included in Supplemental Table S4.
