5-hmC correlates with gene expression in pancreatic cancer and regulates oncogenic pathways. (A) Unsupervised clustering based on gene expression profiling by RNA-seq of pancreatic cancer and pancreatic cancer cells are transcriptionally distinct from controls. (B,C) 5-hmC loci were mapped relative to RefSeq transcripts expressed at different levels in pancreatic cells as obtained from RNA-seq analysis. RefSeq transcripts were divided into two bins based on gene expression level and 5-hmC regions centered on transcription start sites or end sites. (B,C) Enrichment of 5-hmC is seen in highly expressed genes in both controls and cancer cells. (D) Input controls are shown. (E) Differentially expressed genes that are associated with corresponding changes in 5-hmC are grouped in functional pathways by the ingenuity pathway tool.
