Tissue regulation of AS is dominated by neural and muscle. (A) Heatmap and hierarchical clustering of highly regulated AS events (standard deviation of PSIs across samples higher than 20) in widely expressed human genes (events with sufficient read coverage in at least 40 samples). Samples with >80% missing values (i.e., events with read coverage below VLOW) were discarded. Immune-hem: immune-hematopoietic samples. (B) Bar plots showing the number of exons with increased PSI in a specific tissue compared to all other tissue types. (Early Dev) early embryonic stages in mouse (from oocyte to eight-cell stage) and chicken (from Stage X to HH6). (C) RT-PCR validations and corresponding VastDB RNA-seq PSI estimates for exons in Clasp2 (neural, increased PSI) and 1700106N22Rik (testis, decreased PSI).
