VastDB: An atlas of alternative splicing profiles and functional associations in vertebrate cell and tissue types. (Left) vast-tools was used to profile 1478 independent RNA-seq data sets comprising 108 human, 139 mouse, and 61 chicken sets of tissues, cell types, and developmental stages, which produced thousands of AS events of all major types (center). Each AS event possesses a unique and constant event identifier (EventID) across vast-tools and VastDB, allowing profiling of new RNA-seq samples with vast-tools and direct contextualization of interesting events in VastDB. (Right) For each EventID, VastDB provides functional information, including a graphical display of splicing and gene expression levels across samples, sequence features, suggested primers for RT-PCR validation, genomic context through the UCSC Genome Browser, and multiple protein features (e.g., domains, disorder regions, 3D structure). Moreover, EventIDs have a homology annotation interconnecting the AS events across the different VastDB species.
