Transcription-coupled repair of 3meA lesions in a BER-deficient (mag1Δ) strain. (A,B) Fraction of 3meA lesions remaining in a mag1Δ 1- and 2-h sample relative to mag1Δ 0-h control was plotted for bins spanning 501 bp upstream of the transcription start site (TSS), the transcribed region of the gene, and 501 bp downstream from the transcription termination site (TTS) for 5762 yeast genes. (C) No transcription-coupled repair of 7meG lesions in a mag1Δ strain. Same as in part A, except the fraction of 7meG lesions remaining following 2-h repair in a mag1Δ strain was plotted. (D) MMS sensitivity of yeast strains containing single or double mutants in MAG1 or the indicated NER genes (i.e., RAD16, RAD26, and RAD14) when spotted on plates containing the indicated concentrations of MMS.
