Restoration of target gene expression by RNAi in the mir310s- line. (A) Relative expression of the five targets is measured by qRT-PCR in the ovaries of 3-d-old females. In Gal4/UAS-RNAi flies, genes that are de-repressed in the mir310s- line are repressed by RNAi. Assays were taken in triplicate. (*) P < 0.05, (**) P < 0.01, (***) P < 0.001 by Student's t-test. (B) The restoration of target gene expression in the mir310s- line by RNAi. The second column shows the up-regulation of the five targets due to mir310s knockout, and the third column shows the down-regulation due to RNAi of individual targets. The last column gives the restored level relative to the wild-type expression. Note that the tissue distribution of RNAi is under the control of a Gal4, which is itself driven by the mir310s promoter.
