Dhh1-mediated translational control is seemingly conserved from yeast to humans. (A) PARS score distribution analysis of the human mRNA counterparts for the 5′ UTRs and the first 300 nt of the CDS. Axis and smoothing as in Figure 5C. Green box marks nucleotides 60–100. (B) PARS score of the 5′ UTR and the first 300 nt of PTCH1 mRNA. (C) DDX6 drives translation of PTCH1. Western blot, qPCR, and translation rates of PTCH1 after DDX6 silencing. Translation was determined by correcting protein levels by the amount of the corresponding RNA. As a control for equal sample size, tubulin protein levels and HPRT1 mRNA levels were examined. Experiments have been carried out at least three times independently. (**) P < 0.05, t-test.
