Integrating transcriptomic and proteomic data for accurate assembly and annotation of genomes

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Figure 3.
Figure 3.

Reannotation of the An. stephensi genome based on transcriptomic and proteomic evidence. (A) Alignment of RNA-seq data against the 15.4-kb-long mitochondrial genome reveals transcript evidence (colored arrows) for 13 mitochondrial genes, of which seven were also identified at the protein level (red lines). (B) Insertion of a nucleotide base in mitochondrial genome based on RNA-seq evidence. (C) Identification of a novel gene in the AsteI2 and AsteS1 assemblies. (D) Alternate protein start site based on the presence of an upstream N-terminally acetylated peptide.

This Article

  1. Genome Res. 27: 133-144

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