SMARCA4 regulates gene expression and higher-order chromatin structure in proliferating mammary epithelial cells

(A) Western blot of the SMARCA4 protein levels of shSCRAM and shSMARCA4 MCF-10A cells in the noninduced (DOX−) and induced (DOX+) conditions. Lower: Quantification of the Western blot showing ∼85% reduction of SMARCA4 protein levels upon doxycyline induction. (B) Scatter plot showing the log₂ gene expression values for shSMARCA4 and shSCRAM cells. The red and blue dots denote the up- and down-regulated genes between the two conditions, respectively. (C) Heatmap showing the transcripts per million (TPM) expression values of the differentially expressed genes between shSCRAM and shSMARCA4 for each biological replicate. (D,E) Bar graphs showing the −log₁₀ P-values for the REACTOME terms of the (D) 1292 genes that are down-regulated and (E) 176 up-regulated genes upon SMARCA4 knockdown.