Figure 5.
The knock-down of HNRNPC affects CD47 protein localization. (A) Indirect immunophenotyping of membrane-associated CD47 in HEK 293 cells that were treated either with an si-HNRNPC (blue) or with si-Control (red) siRNA. Mean, median, and mode of the Alexa Fluor 488 intensities computed for cells in each transfection set (top), with histograms shown in the bottom panel. (B) Immunofluorescence staining of permeabilized HEK 293 cells with CD47 antibody (left) or nuclear staining with Hoechst (right). Top and bottom panels correspond to cells that were treated with control siRNA and si-HNRNPC, respectively.
