Figure 4.
pRSEM more accurately allocates multimapping reads between genes. (A) Changes of estimated fragment counts between pRSEM and RSEM for pairs of genes overlapping with each other and sharing reads exclusively. Genes that had a fragment count change of less than one were excluded. (B) The numbers of genes for which RSEM (black) and pRSEM (gray) disagreed on expression status. Genes considered were those sharing RNA-seq reads with others but not overlapping with any other genes. The numbers were calculated from 10 RNA-seq replicates from five human and mouse cell lines. Error bars represent one standard deviation.
