Quantitative insertion-site sequencing (QIseq) for high throughput phenotyping of transposon mutants

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Figure 3.
Figure 3.

Distribution and frequency of identified piggyBac insertion sites in the unknown mixed populations (MPs). Distribution of the 254 QIseq insertion sites of the 19 MPs (Supplemental Table S3) shown along the 14 chromosomes; different colors represent different pools. (Inset) Normalized QIseq insertion site: Pools MP1–MP5 were based on 31,000, MP6–MP10 on 62,000, MP11–MP15 on 124,000, and MP15–MP19 248,000 parasites, respectively. The bar plot shows the normalized amount of insertion sites per 31,000 parasites for each unknown MP.

This Article

  1. Genome Res. 26: 980-989

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