Global analysis of transcriptionally engaged yeast RNA polymerase III reveals extended tRNA transcripts

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Figure 3.
Figure 3.

RNAPIII CRAC reveals new RNAPIII transcripts. (A) Putative new RNAPIII transcripts were confirmed by Northern blotting using specific probes against TLT1 (oTWT002) or TLT6 (oTWT007) and the 5.8S rRNA loading control. (B) The Rpo31 (RNAPIII) distribution over the RDN37 gene encoding ribosomal rRNA reveals patterns characteristic of RNAPIII transcription units with the 18S region (red). A common contaminant peak seen in many CRAC analyses is marked with asterisk.

This Article

  1. Published in Advance May 20, 2016, doi: 10.1101/gr.205492.116 Genome Res. 26: 933-944

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