Outline and validation of RNAPIII CRAC. (A) Scheme of Rpo31-HTP fusion (commonly known as Rpc160-HTP) including a C-terminal His6-TEV protease cleavage site-protein A (HTP) tag for CRAC purification. (B) Outline of the CRAC crosslinking protocol. (C) Transcriptome-wide binding profiles for MAF1 (wt) and maf1Δ strains encoding Rpo31-HTP and for the control BY strain expressing untagged Rpo31. Bar diagrams illustrate the percentage of all sequences mapped to each of the RNA classes indicated on the right of the figure. (D) Graphical comparison of the total numbers of reads recovered in RNAPIII CRAC with reads from known RNAPIII transcripts, divided into classes.
