The interaction of PRC2 with RNA or chromatin is mutually antagonistic

Release of PRC2 from chromatin increases binding to RNA. (A) Heat map showing SUZ12 chromatin binding (input subtracted reads/million, according to the scale on the right) 2 kb to either side of the TSS of CpG island–associated genes with significant PRC2 RNA crosslinks. Genes are ordered by PRC2 RNA crosslink density. (B) Average chromatin binding profiles (reads/million) of EZH2 and SUZ12 across gene bodies in Ezh2^fl/fl and Ezh2^Δ/Δ cells. (C, left) CLIP for IgG, FUS, and SUZ12 in Ezh2^fl/fl and Ezh2^Δ/Δ cells. The fold-change in the levels of each protein and crosslinked RNA are indicated below. (Right) Fold-change in RNA crosslinked to FUS and SUZ12 in biological replicate experiments (normalized to protein; mean and SD, n = 3). (D) CLIP for IgG, FUS, and SUZ12/EZH2 in Jarid2^+/+ and Jarid2^−/− cells. Details as for C.