The interaction of PRC2 with RNA or chromatin is mutually antagonistic

PRC2 RNA binding specificity is unchanged in the absence of EZH2 or JARID2. (A) Composite crosslink density profiles for PRC2 in Ezh2^fl/fl, Ezh2^Δ/Δ, Jarid2^+/+, and Jarid2^−/− cells. Details as for Figure 1E. (B) Significant PRC2 crosslinks at the gene Hnrnpa2b1 in Ezh2^fl/fl, Ezh2^Δ/Δ, Jarid2^+/+, and Jarid2^−/− cells. Total RNA reads in Ezh2^fl/fl and Ezh2^Δ/Δ cells are shown below. (C) Dendrogram showing the correlation between composite crosslink density profiles between different iCLIP experiments. (D) Scatter plot comparing the number of significant PRC2 crosslinks per gene between Ezh2^fl/fl and Ezh2^Δ/Δ cells. Overlaid is log₂ fold-change in RNA abundance in Ezh2^Δ/Δ cells compared with Ezh2^fl/fl cells, with shades of red indicating an increase and yellow a decrease, as shown by the scale on the right. (E) Composite crosslink site density profile for SUZ12 in Ezh2^Δ/Δ cells at the set of genes up-regulated in Ezh2^Δ/Δ cells compared with Ezh2^fl/fl cells (Supplemental Fig. S6D, red).