The interaction of PRC2 with RNA or chromatin is mutually antagonistic

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Figure 3.
Figure 3.

SUZ12 directly binds RNA independently of other PRC2 subunits. (A) PAR-CLIP for SUZ12 in Ezh2fl/fl (WT), Ezh2Δ/Δ, and Suz12−/− cells, with and without 4-sU. (B) CLIP for SUZ12 in Eed4 cKO cells before or after the addition of doxycycline to repress expression of MYC-EED4. An arrow marks the SUZ12 RNP. Immunoblots for SUZ12, EZH2, and MYC-EED are shown below. (C) CLIP for JARID2 with anti-Flag antibody in WT (JM8) and JARID2-Flag ESCs. RNase I was titrated at 1, 4, and 20 U/mL. An arrow marks an RNP of the expected molecular weight. (D) CLIP for SUZ12 and IgG in WT (JM8) or Jarid2−/− ESCs. JARID2 and SUZ12 immunoblots are shown below.

This Article

  1. Published in Advance May 9, 2016, doi: 10.1101/gr.197632.115 Genome Res. 26: 896-907

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