The Dionaea hydrolase cocktail is activated synergistically by touch and taste. (A, left) Venn diagram of potential secretome members from an overlay of RNA-seq data (nonstimulated and insect-stimulated glands) and HTS proteomic measurements of secreted fluid from chemically (COR), mechanically, and insect-stimulated traps. Numbers indicate up-regulated (top) and down-regulated (bottom) transcripts. Potential candidates are limited to transcripts that are differentially regulated, contain a proper signal peptide, and are detected at least once using HTS proteomics. (A, right) List of potential secretome members differentially up-regulated after insect stimulation and detected in all three HTS proteomic measurements. (B) qPCR time course of Dionaea marker hydrolases SCPL49 and SAG12 in response to insect, mechanical, and COR stimulation. Both enzymes are up-regulated several thousand-fold after stimulation. Expression at time point 0 h is within statistical noise. (C) Synergistic stimulation of VF CHITINASE I expression in response to an initial mechanical stimulation followed by a chemical stimulus (chitin).
