Topologically associated domains (TADs) are smaller but maintained across the cancer cell genome. (A) Number and average size (in Mb) of TADs identified in normal prostate epithelial cells (PrEC) and prostate cancer cells (PC3 and LNCaP). (B) Chromatin interaction heat maps from PrEC, PC3, and LNCaP cells visualized as two-dimensional interaction matrices in WashU Epigenome Browser. TADs observed in cancer cells are often “merged” in normal cells, and large domains found in normal cells are frequently occupied by more than two domains in cancer cells. The interaction data are aligned with RefSeq genes and CTCF binding sites. An example from Chromosome 1 is shown. (C) Proportion of the genome (%) organized into TADs, associated with domain boundaries, or unorganized chromatin.
