Regulatory properties of active promoters with and without H2BK20ac in GM12878 cells. (A) Clustering of RefSeq promoters by profiles of five histone marks in GM12878 cells: seven major classes. (B) UCSC Genome Browser view of two P3-class promoter regions (black boxes) showing enrichment of H3K27ac but not H2BK20ac. (C) Absolute and cell-type-specific expression, DNase hypersensitivity, and number of flanking enhancers for each of the seven promoter classes in GM12878 cells. Cell-type-specific expression was quantified as fold change relative to the median of 12 cell types. (D) Cell-type-specific expression of top 1000 promoters for each ChIP-seq profile in GM12878 cells. (E) Fold excess above background of TF binding sites in P1 and P3 promoter regions. P7 promoter regions were used as the background set. (Blue dots) TFs enriched more in P1 than in P3 (P-value <0.01, FC > 1.5). (F) Enrichment of lymphocyte-specific functions among genes associated with the seven promoter classes.
