A time- and cost-effective strategy to sequence mammalian Y Chromosomes: an application to the de novo assembly of gorilla Y

(Downloading may take up to 30 seconds. If the slide opens in your browser, select File -> Save As to save it.)

Click on image to view larger version.

Figure 2.
Figure 2.

(A) The global workflow applied for the Y Chromosome assembly (see text for details). Four assemblies in the dotted frame are nested within each other. The best assembly is framed in red. (Orange) Illumina data; (blue) PacBio data. All assemblies were filtered against the reference female genome. The total (including Ns) and unambiguous (non-N, shown in parentheses) lengths are shown. N50 is the contig/scaffold length for which all contigs/scaffolds of that length or longer contain half of the assembly length. (B) Gene and (C) palindrome recovery. The heatmaps show how sequences homologous to 25 human genes, eight human palindromes, and 12 chimpanzee-specific palindromes were recovered in the assemblies (see Methods). Genes lost on the chimpanzee Y are marked with an asterisk.

This Article

  1. Genome Res. 26: 530-540

Preprint Server