Most microRNAs in the single-cell alga Chlamydomonas reinhardtii are produced by Dicer-like 3-mediated cleavage of introns and untranslated regions of coding RNAs

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Figure 3.
Figure 3.

Effect of dcl3 mutation on C. reinhardtii small RNA population. (A) Size-distribution histograms of sRNAs from the parental line A4-1 and its derivative dcl3-1 mutant expressed as the number of counted reads of a given size per million (CPM) of reads matching the C. reinhardtii genome. The percentage of 21-nt sRNAs with their 5′ nucleotide identities is also shown. (B) Size-distribution histograms of nonredundant sRNAs from the parental line A4-1 and its derivative dcl3-1 mutant expressed as CPM of reads matching the C. reinhardtii genome. Two additional replicates per sample, as well as three replicates from the E9-3 parental and dcl3-3 lines, showed the same result.

This Article

  1. Genome Res. 26: 519-529

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