Genome-wide A-to-I RNA editing in fungi independent of ADAR enzymes

A-to-I RNA editing in Neurospora crassa and Fusarium verticillioides. RNA editing in transcripts of the PUK1 orthologs in N. crassa (A) and F. verticillioides (B). The gene structure based on automated annotation (upper) differs from the actual cDNA sequence (lower) identified by RNA-seq analysis. Rectangle boxes are coding regions, and black arrowheads indicate the direction. In N. crassa, NCU03242 is specifically expressed in perithecia, and its UA¹⁶²⁸G stop codon (marked with a black vertical line) corresponding to UA¹⁸³¹G of PUK1 was edited to UGG. In F. verticillioides, RNA editing of UA¹⁸³⁶G in FVEG_01191 (black vertical line) corresponding to UA¹⁸³⁴G of PUK1 was also only observed in perithecia. (C) Numbers of labeled RNA variant sites identified in RNA-seq data of perithecia in F. verticillioides.